• Western blot analysis of extracts of MCF-7 cells, using ITK antibody (STJ114555) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITK antibody (450-620) (STJ114555)

SKU:
STJ114555

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ITK (450-620) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITK
Gene ID: 3702
Uniprot ID: ITK_HUMAN
Immunogen Region: 450-620
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 450-620 of human ITK (NP_005537.3).
Immunogen Sequence: QRGLFAAETLLGMCLDVCEG MAYLEEACVIHRDLAARNCL VGENQVIKVSDFGMTRFVLD DQYTSSTGTKFPVKWASPEV FSFSRYSSKSDVWSFGVLMW EVFSEGKIPYENRSNSEVVE DISTGFRLYKPRLASTHVYQ IMNHCWKERPEDRPAFSRLL RQLAEIAESGL
Tissue Specificity T-cell lines and natural killer cell lines.
Post Translational Modifications Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain. Ubiquitinated.
Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3.
Protein Name Tyrosine-Protein Kinase Itk/Tsk
Interleukin-2-Inducible T-Cell Kinase
Il-2-Inducible T-Cell Kinase
Kinase Emt
T-Cell-Specific Kinase
Tyrosine-Protein Kinase Lyk
Database Links Reactome: R-HSA-202433
Reactome: R-HSA-2871809
Cellular Localisation Cytoplasm
Nucleus
Localizes In The Vicinity Of Cell Surface Receptors In The Plasma Membrane After Receptor Stimulation
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Itk/Tsk antibody
Anti-Interleukin-2-Inducible T-Cell Kinase antibody
Anti-Il-2-Inducible T-Cell Kinase antibody
Anti-Kinase Emt antibody
Anti-T-Cell-Specific Kinase antibody
Anti-Tyrosine-Protein Kinase Lyk antibody
Anti-ITK antibody
Anti-EMT antibody
Anti-LYK antibody

Information sourced from Uniprot.org

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