• Immunohistochemistry analysis of paraffin-embedded human oophoroma using IMP3 Rabbit monoclonal antibody (STJ11103519) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using IMP3 antibody (STJ11103519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-IMP3 antibody (2-194) [S9MR] (STJ11103519)

SKU:
STJ11103519

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IMP3 (2-194) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGF2BP3
Gene ID: 10643
Uniprot ID: IF2B3_HUMAN
Immunogen Region: 2-194
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-194 of human IMP3 (O00425).
Immunogen Sequence: NKLYIGNLSENAAPSDLESI FKDAKIPVSGPFLVKTGYAF VDCPDESWALKAIEALSGKI ELHGKPIEVEHSVPKRQRIR KLQIRNIPPHLQWEVLDSLL VQYGVVESCEQVNTDSETAV VNVTYSSKDQARQALDKLNG FQLENFTLKVAYIPDEMAAQ QNPLQQPRGRRGLGQRGSSR QGSPGSVSKQKPC
Tissue Specificity Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
Function RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
Protein Name Insulin-Like Growth Factor 2 Mrna-Binding Protein 3
Igf2 Mrna-Binding Protein 3
Imp-3
Igf-Ii Mrna-Binding Protein 3
Kh Domain-Containing Protein Overexpressed In Cancer
Hkoc
Vickz Family Member 3
Database Links Reactome: R-HSA-428359
Cellular Localisation Nucleus
Cytoplasm
P-Body
Stress Granule
Found In Lamellipodia Of The Leading Edge
In The Perinuclear Region
And Beneath The Plasma Membrane
The Subcytoplasmic Localization Is Cell Specific And Regulated By Cell Contact And Growth
Localized At The Connecting Piece And The Tail Of The Spermatozoa
Colocalized With Cd44 Mrna In Rnp Granules
In Response To Cellular Stress
Such As Oxidative Stress
Recruited To Stress Granules
Alternative Antibody Names Anti-Insulin-Like Growth Factor 2 Mrna-Binding Protein 3 antibody
Anti-Igf2 Mrna-Binding Protein 3 antibody
Anti-Imp-3 antibody
Anti-Igf-Ii Mrna-Binding Protein 3 antibody
Anti-Kh Domain-Containing Protein Overexpressed In Cancer antibody
Anti-Hkoc antibody
Anti-Vickz Family Member 3 antibody
Anti-IGF2BP3 antibody
Anti-IMP3 antibody
Anti-KOC1 antibody
Anti-VICKZ3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance