| Host: | Rabbit |
| Applications: | WB/IF/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Interleukin-10 receptor subunit alpha (462-511 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IF 1:200-1:1000 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IL10RA |
| Gene ID: | 3587 |
| Uniprot ID: | I10R1_HUMAN |
| Immunogen Region: | 462-511 aa |
| Specificity: | IL-10R Alpha Polyclonal Antibody detects endogenous levels of IL-10R Alpha protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human IL-10R alpha at the amino acid range 462-511 |
| Post Translational Modifications | Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation. Ubiquitinated by BTRC.ubiquitination leads to endocytosis and subsequent degradation of IL10RA. |
| Function | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy. |
| Protein Name | Interleukin-10 Receptor Subunit Alpha Il-10 Receptor Subunit Alpha Il-10r Subunit Alpha Il-10ra Cdw210a Interleukin-10 Receptor Subunit 1 Il-10r Subunit 1 Il-10r1 Cd Antigen Cd210 |
| Database Links | Reactome: R-HSA-6783783 Reactome: R-HSA-9725371 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cytoplasm |
| Alternative Antibody Names | Anti-Interleukin-10 Receptor Subunit Alpha antibody Anti-Il-10 Receptor Subunit Alpha antibody Anti-Il-10r Subunit Alpha antibody Anti-Il-10ra antibody Anti-Cdw210a antibody Anti-Interleukin-10 Receptor Subunit 1 antibody Anti-Il-10r Subunit 1 antibody Anti-Il-10r1 antibody Anti-Cd Antigen Cd210 antibody Anti-IL10RA antibody Anti-IL10R antibody |
Information sourced from Uniprot.org