| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IGFBP5 |
| Gene ID: | 3488 |
| Uniprot ID: | IBP5_HUMAN |
| Immunogen Region: | 68-167 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 68-167 of human IGFBP5 (NP_000590.1). |
| Immunogen Sequence: | GVYTERCAQGLRCLPRQDEE KPLHALLHGRGVCLNEKSYR EQVKIERDSREHEEPTTSEM AEETYSPKIFRPKHTRISEL KAEAVKKDRRKKLTQSKFVG |
| Tissue Specificity | Osteosarcoma, and at lower levels in liver, kidney and brain. |
| Post Translational Modifications | Cleaved by C1S in extracellular space. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells. Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling. Plays also a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. Acts itself as a growth factor that can act independently of IGFs to regulate bone formation. Acts as a ligand for the ROR1 receptor which triggers formation of ROR1/HER2 heterodimer to enhance CREB oncogenic signaling. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 5 Ibp-5 Igf-Binding Protein 5 Igfbp-5 |
| Database Links | Reactome: R-HSA-381426 Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted Cytoplasm Nucleus |
| Alternative Antibody Names | Anti-Insulin-Like Growth Factor-Binding Protein 5 antibody Anti-Ibp-5 antibody Anti-Igf-Binding Protein 5 antibody Anti-Igfbp-5 antibody Anti-IGFBP5 antibody Anti-IBP5 antibody |
Information sourced from Uniprot.org