• Western blot analysis of extracts of various cell lines, using IGFBP2 antibody (STJ11103643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using IGFBP2 Rabbit monoclonal antibody (STJ11103643) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using IGFBP2 rabbit monoclonal antibody (STJ11103643) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using IGFBP2 antibody (STJ11103643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-IGFBP2 antibody (226-325) [S3MR] (STJ11103643)

SKU:
STJ11103643

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-IGFBP2 (226-325) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGFBP2
Gene ID: 3485
Uniprot ID: IBP2_HUMAN
Immunogen Region: 226-325
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 226-325 of human IGFBP2 (P18065).
Immunogen Sequence: PCQQELDQVLERISTMRLPD ERGPLEHLYSLHIPNCDKHG LYNLKQCKMSLNGQRGECWC VNPNTGKLIQGAPTIRGDPE CHLFYNEQQEARGVHTQRMQ
Post Translational Modifications O-glycosylated.
Function Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Protein Name Insulin-Like Growth Factor-Binding Protein 2
Ibp-2
Igf-Binding Protein 2
Igfbp-2
Database Links Reactome: R-HSA-381426
Cellular Localisation Secreted
Alternative Antibody Names Anti-Insulin-Like Growth Factor-Binding Protein 2 antibody
Anti-Ibp-2 antibody
Anti-Igf-Binding Protein 2 antibody
Anti-Igfbp-2 antibody
Anti-IGFBP2 antibody
Anti-BP2 antibody
Anti-IBP2 antibody

Information sourced from Uniprot.org

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