• Immunoprecipitation analysis of 200 Mu g extracts of K562 cells using 1 Mu g IGF2BP1/IMP1 antibody (STJ115542). Western blot was performed from the immunoprecipitate using IGF2BP1/IMP1 antibody (STJ115542) at a dilution of 1:1000.
  • Western blot analysis of various lysates using IGF2BP1/IMP1 Rabbit polyclonal antibody (STJ115542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-IGF2BP1 antibody (478-577) (STJ115542)

SKU:
STJ115542

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IGF2BP1 (478-577) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IGF2BP1
Gene ID: 10642
Uniprot ID: IF2B1_HUMAN
Immunogen Region: 478-577
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 478-577 of human IGF2BP1/IMP1 (NP_006537.3).
Immunogen Sequence: NFFGPKEEVKLETHIRVPAS AAGRVIGKGGKTVNELQNLT AAEVVVPRDQTPDENDQVIV KIIGHFYASQMAQRKIRDIL AQVKQQHQKGQSNQAQARRK
Tissue Specificity Mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus (at protein level). Also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta (at protein level). Expressed in various cancers, including testis and lung cancers (at protein level), as well as kidney, prostate and trachea cancers.
Post Translational Modifications Phosphorylated. Phosphorylation may impair association with ACTB mRNA and hence abolishes translational repression.
Function RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability. Plays a direct role in the transport and translation of transcripts required for axonal regeneration in adult sensory neurons. Regulates localized beta-actin/ACTB mRNA translation, a crucial process for cell polarity, cell migration and neurite outgrowth. Co-transcriptionally associates with the ACTB mRNA in the nucleus. This binding involves a conserved 54-nucleotide element in the ACTB mRNA 3'-UTR, known as the 'zipcode'. The RNP thus formed is exported to the cytoplasm, binds to a motor protein and is transported along the cytoskeleton to the cell periphery. During transport, prevents ACTB mRNA from being translated into protein. When the RNP complex reaches its destination near the plasma membrane, IGF2BP1 is phosphorylated. This releases the mRNA, allowing ribosomal 40S and 60S subunits to assemble and initiate ACTB protein synthesis. Monomeric ACTB then assembles into the subcortical actin cytoskeleton. During neuronal development, key regulator of neurite outgrowth, growth cone guidance and neuronal cell migration, presumably through the spatiotemporal fine tuning of protein synthesis, such as that of ACTB. May regulate mRNA transport to activated synapses. Binds to and stabilizes ABCB1/MDR-1 mRNA. During interstinal wound repair, interacts with and stabilizes PTGS2 transcript. PTGS2 mRNA stabilization may be crucial for colonic mucosal wound healing. Binds to the 3'-UTR of IGF2 mRNA by a mechanism of cooperative and sequential dimerization and regulates IGF2 mRNA subcellular localization and translation. Binds to MYC mRNA, in the coding region instability determinant (CRD) of the open reading frame (ORF), hence preventing MYC cleavage by endonucleases and possibly microRNA targeting to MYC-CRD. Binding to MYC mRNA is enhanced by m6A-modification of the CRD. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to the oncofetal H19 transcript and to the neuron-specific TAU mRNA and regulates their localizations. Binds to and stabilizes BTRC/FBW1A mRNA. Binds to the adenine-rich autoregulatory sequence (ARS) located in PABPC1 mRNA and represses its translation. PABPC1 mRNA-binding is stimulated by PABPC1 protein. Prevents BTRC/FBW1A mRNA degradation by disrupting microRNA-dependent interaction with AGO2. Promotes the directed movement of tumor-derived cells by fine-tuning intracellular signaling networks. Binds to MAPK4 3'-UTR and inhibits its translation. Interacts with PTEN transcript open reading frame (ORF) and prevents mRNA decay. This combined action on MAPK4 (down-regulation) and PTEN (up-regulation) antagonizes HSPB1 phosphorylation, consequently it prevents G-actin sequestration by phosphorylated HSPB1, allowing F-actin polymerization. Hence enhances the velocity of cell migration and stimulates directed cell migration by PTEN-modulated polarization. Interacts with Hepatitis C virus (HCV) 5'-UTR and 3'-UTR and specifically enhances translation at the HCV IRES, but not 5'-cap-dependent translation, possibly by recruiting eIF3. Interacts with HIV-1 GAG protein and blocks the formation of infectious HIV-1 particles. Reduces HIV-1 assembly by inhibiting viral RNA packaging, as well as assembly and processing of GAG protein on cellular membranes. During cellular stress, such as oxidative stress or heat shock, stabilizes target mRNAs that are recruited to stress granules, including CD44, IGF2, MAPK4, MYC, PTEN, RAPGEF2 and RPS6KA5 transcripts.
Protein Name Insulin-Like Growth Factor 2 Mrna-Binding Protein 1
Igf2 Mrna-Binding Protein 1
Imp-1
Imp1
Coding Region Determinant-Binding Protein
Crd-Bp
Igf-Ii Mrna-Binding Protein 1
Vickz Family Member 1
Zipcode-Binding Protein 1
Zbp-1
Database Links Reactome: R-HSA-428359
Reactome: R-HSA-5687128
Cellular Localisation Nucleus
Cytoplasm
Perinuclear Region
P-Body
Stress Granule
Cell Projection
Lamellipodium
Dendrite
Dendritic Spine
Growth Cone
Filopodium
Axon
In The Nucleus
Located In Discrete Foci
Coinciding With The Sites Of Actb Transcription
In The Cytoplasm
Localizes In Cytoplasmic Mrnp Granules
Colocalizes With Microtubules In Growth Cone Filopodia And Along Neurites In Neuronal Cells
Cytoplasmic Colocalization With Actb Mrna Is Partially Lost At The Cell Periphery
Suggesting Release Of The Transcript
In Neuronal Processes
Exhibits Fast Retrograde And Anterograde Movements
When Associated With Actb Mrna
This Motility Is Lost When The Association Is Inhibited
In Hippocampal Neurons
Predominantly Located Within Dendrites
Particularly At Dendritic Branching Points In Young Cells
Compared To Axons
In Axons
Predominantly Found In Axonal Branches And Their Growth Cones
In Motile Cells
Such As Migrating Fibroblasts
Localizes To Leading Edges Where It Colocalizes With Microtubules And Microfilaments And To Retracting Tails
Dendritic Levels Are Regulated By Neuronal Activity And Glutaminergic Signals: They Are Increased By Kcl-Induced Depolarization
Which Induces Rapid Efflux From The Cell Body Into Dendrites
And Decreased By The Nmda Receptor Agonist
Transported Towards The Leading Edge Into The Cortical Region Of The Lamellipodia Where It Is Connected To Microfilaments
In Response To Cellular Stress
Such As Oxidative Stress Or Heat Shock
Recruited To Stress Granules
But Not To Processing Bodies
Alternative Antibody Names Anti-Insulin-Like Growth Factor 2 Mrna-Binding Protein 1 antibody
Anti-Igf2 Mrna-Binding Protein 1 antibody
Anti-Imp-1 antibody
Anti-Imp1 antibody
Anti-Coding Region Determinant-Binding Protein antibody
Anti-Crd-Bp antibody
Anti-Igf-Ii Mrna-Binding Protein 1 antibody
Anti-Vickz Family Member 1 antibody
Anti-Zipcode-Binding Protein 1 antibody
Anti-Zbp-1 antibody
Anti-IGF2BP1 antibody
Anti-CRDBP antibody
Anti-VICKZ1 antibody
Anti-ZBP1 antibody

Information sourced from Uniprot.org

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