• Western blot analysis of extracts of various cell lines, using HYAL3 antibody (STJ29091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HYAL3 antibody (198-417) (STJ29091)

SKU:
STJ29091

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HYAL3 (198-417) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HYAL3
Gene ID: 8372
Uniprot ID: HYAL3_HUMAN
Immunogen Region: 198-417
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 198-417 of human HYAL3 (NP_003540.2).
Immunogen Sequence: GFYHYPACGNGWHSMASNYT GRCHAATLARNTQLHWLWAA SSALFPSIYLPPRLPPAHHQ AFVRHRLEEAFRVALVGHRH PLPVLAYVRLTHRRSGRFLS QDDLVQSIGVSAALGAAGVV LWGDLSLSSSEEECWHLHDY LVDTLGPYVINVTRAAMACS HQRCHGHGRCARRDPGQMEA FLHLWPDGSLGDWKSFSCHC YWGWAGPTCQEPRPGPKEAV
Tissue Specificity Expressed in sperm. Highly expressed in epidermis of the skin, where it is expressed intracellularily in the deep horny layer (at protein level). Bone marrow, testis and kidney.
Post Translational Modifications N-glycosylated.
Function Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm. Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage. Has no hyaluronidase activity in embryonic fibroblasts in vitro. Has no hyaluronidase activity in granulosa cells in vitro.
Protein Name Hyaluronidase-3
Hyal-3
Hyaluronoglucosaminidase-3
Lung Carcinoma Protein 3
Luca-3
Database Links Reactome: R-HSA-2024101
Reactome: R-HSA-2160916
Cellular Localisation Secreted
Cell Membrane
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Endoplasmic Reticulum
Early Endosome
Mostly Present In Low-Density Vesicles
Low Levels In Higher Density Vesicles Of Late Endosomes And Lysosomes
Localized In Punctate Cytoplasmic Vesicles And In Perinuclear Structures
But Does Not Colocalize With Lamp1
Localized On The Plasma Membrane Over The Acrosome And On The Surface Of The Midpiece Of The Sperm Tail
Alternative Antibody Names Anti-Hyaluronidase-3 antibody
Anti-Hyal-3 antibody
Anti-Hyaluronoglucosaminidase-3 antibody
Anti-Lung Carcinoma Protein 3 antibody
Anti-Luca-3 antibody
Anti-HYAL3 antibody
Anti-LUCA3 antibody

Information sourced from Uniprot.org

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