• Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g HTRA2 antibody (STJ117077). Western blot was performed from the immunoprecipitate using HTRA2 antibody (STJ117077) at a dilution of 1:1000.
  • Western blot analysis of lysates from MCF7 cells, using HTRA2 Rabbit polyclonal antibody (STJ117077) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HTRA2 antibody (334-458) (STJ117077)

SKU:
STJ117077

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTRA2
Gene ID: 27429
Uniprot ID: HTRA2_HUMAN
Immunogen Region: 334-458
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 334-458 of human HTRA2 (NP_037379.1).
Immunogen Sequence: PSDRLREFLHRGEKKNSSSG ISGSQRRYIGVMMLTLSPSI LAELQLREPSFPDVQHGVLI HKVILGSPAHRAGLRPGDVI LAIGEQMVQNAEDVYEAVRT QSQLAVQIRRGRETLTLYVT PEVTE
Tissue Specificity Isoform 1: Ubiquitously expressed.
Post Translational Modifications Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. Autoproteolytically activated.
Function Isoform 1: Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity.or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2: Seems to be proteolytically inactive.
Protein Name Serine Protease Htra2 - Mitochondrial
High Temperature Requirement Protein A2
Htra2
Omi Stress-Regulated Endoprotease
Serine Protease 25
Serine Proteinase Omi
Database Links Reactome: R-HSA-9837999
Cellular Localisation Mitochondrion Intermembrane Space
Mitochondrion Membrane
Single-Pass Membrane Protein
Predominantly Present In The Intermembrane Space
Released Into The Cytosol Following Apoptotic Stimuli
Such As Uv Treatment
And Stimulation Of Mitochondria With Caspase-8 Truncated Bid/Tbid
Isoform 1: Endoplasmic Reticulum
Alternative Antibody Names Anti-Serine Protease Htra2 - Mitochondrial antibody
Anti-High Temperature Requirement Protein A2 antibody
Anti-Htra2 antibody
Anti-Omi Stress-Regulated Endoprotease antibody
Anti-Serine Protease 25 antibody
Anti-Serine Proteinase Omi antibody
Anti-HTRA2 antibody
Anti-OMI antibody
Anti-PRSS25 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance