Anti-HTATSF1 antibody (Internal) (STJ71288)

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STJ71288-100
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-HTATSF1 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:16000.
WB: Approx 140kDa band observed in lysates of cell line Daudi (calculated MW of 85.9kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literatu
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HTATSF1
Gene ID: 27336
Uniprot ID: HTSF1_HUMAN
Immunogen Region: Internal
Accession Number: NP_055315.2
Immunogen Sequence: QELYGDGKDGDTQTD
Post Translational Modifications Phosphorylation at Ser-748 by CK2 during S-phase in response to DNA damage promotes interaction with TOPBP1 and double-strand break (DSB) repair via homologous recombination.
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, HTATSF1 is required to stabilize the branchpoint-interacting stem loop. HTATSF1 is displaced from the 17S U2 SnRNP complex before the stable addition of the 17S U2 SnRNP complex to the spliceosome, destabilizing the branchpoint-interacting stem loop and allowing to probe intron branch site sequences. Also acts as a regulator of transcriptional elongation, possibly by mediating the reciprocal stimulatory effect of splicing on transcriptional elongation. Involved in double-strand break (DSB) repair via homologous recombination in S-phase by promoting the recruitment of TOPBP1 to DNA damage sites. Mechanistically, HTATSF1 is (1) recruited to DNA damage sites in S-phase via interaction with poly-ADP-ribosylated RPA1 and (2) phosphorylated by CK2, promoting recruitment of TOPBP1, thereby facilitating RAD51 nucleofilaments formation and RPA displacement, followed by homologous recombination. (Microbial infection) In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.
Protein Name 17s U2 Snrnp Complex Component Htatsf1
Hiv Tat-Specific Factor 1
Tat-Sf1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Chromosome
Recruited To Dna Damage Sites During S-Phase Following Interaction With Poly-Adp-Ribosylated Rpa1
Alternative Antibody Names Anti-17s U2 Snrnp Complex Component Htatsf1 antibody
Anti-Hiv Tat-Specific Factor 1 antibody
Anti-Tat-Sf1 antibody
Anti-HTATSF1 antibody

Information sourced from Uniprot.org