Western blot analysis of lysates from U-251MG cells, using HS3ST3A1 Rabbit polyclonal antibody (STJ118902) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HS3ST3A1 antibody (44-140) (STJ118902)

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STJ118902
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HS3ST3A1
Gene ID: 9955
Uniprot ID: HS3SA_HUMAN
Immunogen Region: 44-140
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 44-140 of human HS3ST3A1 (NP_006033.1).
Immunogen Sequence: ERCQTLSGPVVGLSGGGEEA GAPGGGVLAGGPRELAVWPA AAQRKRLLQLPQWRRRRPPA PRDDGEEAAWEEESPGLSGG PGGSGAGSTVAEAPPGT
Tissue Specificity Ubiquitous. Most abundant in heart and placenta, followed by liver and kidney.
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. Unlike HS3ST1/3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.
Protein Name Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3a1
Heparan Sulfate D-Glucosaminyl 3-O-Sulfotransferase 3a1
3-Ost-3a
Heparan Sulfate 3-O-Sulfotransferase 3a1
H3-Ost-3a
Database Links Reactome: R-HSA-2022928
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Heparan Sulfate Glucosamine 3-O-Sulfotransferase 3a1 antibody
Anti-Heparan Sulfate D-Glucosaminyl 3-O-Sulfotransferase 3a1 antibody
Anti-3-Ost-3a antibody
Anti-Heparan Sulfate 3-O-Sulfotransferase 3a1 antibody
Anti-H3-Ost-3a antibody
Anti-HS3ST3A1 antibody
Anti-3OST3A1 antibody
Anti-HS3ST3A antibody
Anti-UNQ2551 antibody
Anti-PRO6180 antibody

Information sourced from Uniprot.org

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