• Confocal imaging of U-2 0S cells using hnRNP K Rabbit monoclonal antibody (STJ11101255, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded rat spleen tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded rat colon tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded mouse testis tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded mouse spleen tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded mouse liver tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded mouse colon tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded human tonsil tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded Human lung adenocarcinoma tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded human colon tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of hnRNP K in paraffin-embedded human cervix cancer tissue using hnRNP K Rabbit monoclonal antibody (STJ11101255) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of extracts of various cell lines, using hnRNP K antibody (STJ11101255) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HNRNPK antibody (1-100) [S5MR] (STJ11101255)

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STJ11101255

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-hnRNP K (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPK
Gene ID: 3190
Uniprot ID: HNRPK_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human hnRNP K (P61978).
Immunogen Sequence: METEQPEETFPNTETNGEFG KRPAEDMEEEQAFKRSRNTD EMVELRILLQSKNAGAVIGK GGKNIKALRTDYNASVSVPD SSGPERILSISADIETIGEI
Post Translational Modifications Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions. Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Function One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation.
Protein Name Heterogeneous Nuclear Ribonucleoprotein K
Hnrnp K
Transformation Up-Regulated Nuclear Protein
Tunp
Database Links Reactome: R-HSA-4570464
Reactome: R-HSA-72163
Reactome: R-HSA-72203
Reactome: R-HSA-9610379
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Cell Projection
Podosome
Recruited To P53/Tp53-Responsive Promoters
In The Presence Of Functional P53/Tp53
In Case Of Asfv Infection
There Is A Shift In The Localization Which Becomes Predominantly Nuclear
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein K antibody
Anti-Hnrnp K antibody
Anti-Transformation Up-Regulated Nuclear Protein antibody
Anti-Tunp antibody
Anti-HNRNPK antibody
Anti-HNRPK antibody

Information sourced from Uniprot.org

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