• Western blot analysis of extracts of HT-29 cells, using HN1 antibody (STJ118563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded Rat testis using HN1 antibody (STJ118563) at dilution of 1:150 (20x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using HN1 antibody (STJ118563) at dilution of 1:150 (20x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human tonsil using HN1 antibody (STJ118563) at dilution of 1:150 (20x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-HN1 antibody (1-154) (STJ118563)

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STJ118563

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HN1 (1-154) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: JPT1
Gene ID: 51155
Uniprot ID: JUPI1_HUMAN
Immunogen Region: 1-154
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human HN1 (NP_057269.1).
Immunogen Sequence: MTTTTTFKGVDPNSRNSSRV LRPPGGGSNFSLGFDEPTEQ PVRKNKMASNIFGTPEENQA SWAKSAGAKSSGGREDLESS GLQRRNSSEASSGDFLDLKG EGDIHENVDTDLPGSLGQSE EKPVPAAPVPSPVAPAPVPS RRNPPGGKSSLVLG
Tissue Specificity Expressed in testis, skeletal muscle, thymus, prostate, colon, peripheral blood cells, brain and placenta.
Function Modulates negatively AKT-mediated GSK3B signaling. Induces CTNNB1 'Ser-33' phosphorylation and degradation through the suppression of the inhibitory 'Ser-9' phosphorylation of GSK3B, which represses the function of the APC:CTNNB1:GSK3B complex and the interaction with CDH1/E-cadherin in adherent junctions. Plays a role in the regulation of cell cycle and cell adhesion. Has an inhibitory role on AR-signaling pathway through the induction of receptor proteasomal degradation.
Protein Name Jupiter Microtubule Associated Homolog 1
Androgen-Regulated Protein 2
Hematological And Neurological Expressed 1 Protein Cleaved Into - Jupiter Microtubule Associated Homolog 1 - N-Terminally Processed
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Jupiter Microtubule Associated Homolog 1 antibody
Anti-Androgen-Regulated Protein 2 antibody
Anti-Hematological And Neurological Expressed 1 Protein Cleaved Into - Jupiter Microtubule Associated Homolog 1 - N-Terminally Processed antibody
Anti-JPT1 antibody
Anti-ARM2 antibody
Anti-HN1 antibody

Information sourced from Uniprot.org

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