• Western blot analysis of extracts of various cell lines, using HMGN2 antibody (STJ27909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-HMGN2 antibody (1-90) (STJ27909)

SKU:
STJ27909

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMGN2 (1-90) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGN2
Gene ID: 3151
Uniprot ID: HMGN2_HUMAN
Immunogen Region: 1-90
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human HMGN2 (NP_005508.1).
Immunogen Sequence: MPKRKAEGDAKGDKAKVKDE PQRRSARLSAKPAPPKPEPK PKKAPAKKGEKVPKGKKGKA DAGKEGNNPAENGDAKTDQA QKAEGAGDAK
Post Translational Modifications Phosphorylation favors cytoplasmic localization.
Function Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation.
Protein Name Non-Histone Chromosomal Protein Hmg-17
High Mobility Group Nucleosome-Binding Domain-Containing Protein 2
Cellular Localisation Nucleus
Cytoplasm
Cytoplasmic Enrichment Upon Phosphorylation
Alternative Antibody Names Anti-Non-Histone Chromosomal Protein Hmg-17 antibody
Anti-High Mobility Group Nucleosome-Binding Domain-Containing Protein 2 antibody
Anti-HMGN2 antibody
Anti-HMG17 antibody

Information sourced from Uniprot.org

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