| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HLA-F (22-190) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HLA-F |
| Gene ID: |
3134 |
| Uniprot ID: |
HLAF_HUMAN |
| Immunogen Region: |
22-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human HLA-F (NP_001091949.1). |
| Immunogen Sequence: |
GSHSLRYFSTAVSRPGRGEP RYIAVEYVDDTQFLRFDSDA AIPRMEPREPWVEQEGPQYW EWTTGYAKANAQTDRVALRN LLRRYNQSEAGSHTLQGMNG CDMGPDGRLLRGYHQHAYDG KDYISLNEDLRSWTAADTVA QITQRFYEAEEYAEEFRTYL EGECLELLR |
| Tissue Specificity | Expressed in resting B cells (at protein level). Expressed in secondary lymphoid organs rich in B and T cells such as the tonsils, spleen, and thymus (at protein level). Expressed in the endothelial cells of the tonsils. Expressed on activated lymphoid cells including B cells, NK cells, CD4+ T cells and memory T cells (at protein level). Expressed in motor neurons of spinal cord. |
| Post Translational Modifications | N-glycosylated. |
| Function | Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors. Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production. Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions. May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses. May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity. |
| Protein Name | Hla Class I Histocompatibility Antigen - Alpha Chain FCda12Hla F AntigenLeukocyte Antigen FMhc Class I Antigen F |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-1236977Reactome: R-HSA-198933Reactome: R-HSA-877300Reactome: R-HSA-909733Reactome: R-HSA-9705671Reactome: R-HSA-983170 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEarly Endosome MembraneLysosome MembraneFor Cross-Presentation Transits From The Cell Surface Through Endosomal Pathway To LysosomesWhere The Peptide Is Generated From Internalized Exogenous Antigen |
| Alternative Antibody Names | Anti-Hla Class I Histocompatibility Antigen - Alpha Chain F antibodyAnti-Cda12 antibodyAnti-Hla F Antigen antibodyAnti-Leukocyte Antigen F antibodyAnti-Mhc Class I Antigen F antibodyAnti-HLA-F antibodyAnti-HLA-5.4 antibodyAnti-HLAF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
