| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HLA-F |
| Gene ID: | 3134 |
| Uniprot ID: | HLAF_HUMAN |
| Immunogen Region: | 22-190 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human HLA-F (NP_001091949.1). |
| Immunogen Sequence: | GSHSLRYFSTAVSRPGRGEP RYIAVEYVDDTQFLRFDSDA AIPRMEPREPWVEQEGPQYW EWTTGYAKANAQTDRVALRN LLRRYNQSEAGSHTLQGMNG CDMGPDGRLLRGYHQHAYDG KDYISLNEDLRSWTAADTVA QITQRFYEAEEYAEEFRTYL EGECLELLR |
| Tissue Specificity | Expressed in resting B cells (at protein level). Expressed in secondary lymphoid organs rich in B and T cells such as the tonsils, spleen, and thymus (at protein level). Expressed in the endothelial cells of the tonsils. Expressed on activated lymphoid cells including B cells, NK cells, CD4+ T cells and memory T cells (at protein level). Expressed in motor neurons of spinal cord. |
| Post Translational Modifications | N-glycosylated. |
| Function | Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors. Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production. Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions. May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses. May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity. |
| Protein Name | Hla Class I Histocompatibility Antigen - Alpha Chain F Cda12 Hla F Antigen Leukocyte Antigen F Mhc Class I Antigen F |
| Database Links | Reactome: R-HSA-1236974 Reactome: R-HSA-1236977 Reactome: R-HSA-198933 Reactome: R-HSA-877300 Reactome: R-HSA-909733 Reactome: R-HSA-9705671 Reactome: R-HSA-983170 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Early Endosome Membrane Lysosome Membrane For Cross-Presentation Transits From The Cell Surface Through Endosomal Pathway To Lysosomes Where The Peptide Is Generated From Internalized Exogenous Antigen |
| Alternative Antibody Names | Anti-Hla Class I Histocompatibility Antigen - Alpha Chain F antibody Anti-Cda12 antibody Anti-Hla F Antigen antibody Anti-Leukocyte Antigen F antibody Anti-Mhc Class I Antigen F antibody Anti-HLA-F antibody Anti-HLA-5.4 antibody Anti-HLAF antibody |
Information sourced from Uniprot.org