| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HIC1 (180-430) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HIC1 |
| Gene ID: |
3090 |
| Uniprot ID: |
HIC1_HUMAN |
| Immunogen Region: |
180-430 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 180-430 of human HIC1 (NP_001091672.1). |
| Immunogen Sequence: |
AATPVIQACYPSPVGPPPPP AAEPPSGPEAAVNTHCAELY ASGPGPAAALCASERRCSPL CGLDLSKKSPPGSAAPERPL AERELPPRPDSPPSAGPAAY KEPPLALPSLPPLPFQKLEE AAPPSDPFRGGSGSPGPEPP GRPDGPSLLYRWMKHEPGLG SYGDELGRERGSPSERCEER GGDAAVSPGGPPLGLAPPPR YPGSLDGPGAGGDGDDYKSS SEETGSSEDPSPPGGHLEG |
| Tissue Specificity | Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells. |
| Post Translational Modifications | Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1. Sumoylated on Lys-333 by a PIAS family member, which enhances interaction with MTA1, positively regulates transcriptional repression activity and is enhanced by HDAC4. |
| Function | Transcriptional repressor. Recognizes and binds to the consensus sequence '5-CGNGCGGGGCACACC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription of ACKR3, FGFBP1 and EFNA1. |
| Protein Name | Hypermethylated In Cancer 1 ProteinHic-1Zinc Finger And Btb Domain-Containing Protein 29 |
| Database Links | Reactome: R-HSA-3232118 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Hypermethylated In Cancer 1 Protein antibodyAnti-Hic-1 antibodyAnti-Zinc Finger And Btb Domain-Containing Protein 29 antibodyAnti-HIC1 antibodyAnti-ZBTB29 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
