| Host: |
Goat |
| Applications: |
Pep-ELISA |
| Reactivity: |
Human/Mouse/Rat/Dog/Horse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-HEM1 (Internal) is suitable for use in ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
ELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
NCKAP1L |
| Gene ID: |
3071 |
| Uniprot ID: |
NCKPL_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_005328.2 |
| Immunogen Sequence: |
RHTENVTKTKTPE |
| Function | Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages. Component of the WAVE2 complex which signals downstream of RAC to stimulate F-actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells. Within the WAVE2 complex, enables the cortical actin network to restrain excessive degranulation and granule release by T-cells. Required for efficient T-lymphocyte and neutrophil migration. Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin. Also involved in mechanisms WAVE-independent to regulate myosin and actin polymerization during neutrophil chemotaxis. In T-cells, required for proper mechanistic target of rapamycin complex 2 (mTORC2)-dependent AKT phosphorylation, cell proliferation and cytokine secretion, including that of IL2 and TNF. |
| Protein Name | Nck-Associated Protein 1-LikeHematopoietic Protein 1Membrane-Associated Protein Hem-1 |
| Database Links | Reactome: R-HSA-2029482Reactome: R-HSA-4420097Reactome: R-HSA-5663213Reactome: R-HSA-6798695Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinCytoplasmic SideCytoplasmLocalizes To The Leading Edge Of Polarized Neutrophils |
| Alternative Antibody Names | Anti-Nck-Associated Protein 1-Like antibodyAnti-Hematopoietic Protein 1 antibodyAnti-Membrane-Associated Protein Hem-1 antibodyAnti-NCKAP1L antibodyAnti-HEM1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
