• Confocal imaging of K-562 cells using HDAC9 Rabbit monoclonal antibody (STJ11101796, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using HDAC9 Rabbit monoclonal antibody (STJ11101796, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of HDAC9 in paraffin-embedded human colon carcinoma using HDAC9 Rabbit monoclonal antibody (STJ11101796) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HDAC9 in paraffin-embedded rat testis using HDAC9 Rabbit monoclonal antibody (STJ11101796) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using HDAC9 Rabbit monoclonal antibody (STJ11101796) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-HDAC9 antibody (1-100) [S6MR] (STJ11101796)

SKU:
STJ11101796

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HDAC9 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC9
Gene ID: 9734
Uniprot ID: HDAC9_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC9 (Q9UKV0).
Immunogen Sequence: MHSMISSVDVKSEVPVGLEP ISPLDLRTDLRMMMPVVDPV VREKQLQQELLLIQQQQQIQ KQLLIAEFQKQHENLTRQHQ AQLQEHIKELLAIKQQQELL
Tissue Specificity Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
Post Translational Modifications Phosphorylated on Ser-220 and Ser-450.which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240.which impairs nuclear accumulation. Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Sumoylated.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
Protein Name Histone Deacetylase 9
Hd9
Histone Deacetylase 7b
Hd7
Hd7b
Histone Deacetylase-Related Protein
Mef2-Interacting Transcription Repressor Mitr
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-350054
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Histone Deacetylase 9 antibody
Anti-Hd9 antibody
Anti-Histone Deacetylase 7b antibody
Anti-Hd7 antibody
Anti-Hd7b antibody
Anti-Histone Deacetylase-Related Protein antibody
Anti-Mef2-Interacting Transcription Repressor Mitr antibody
Anti-HDAC9 antibody
Anti-HDAC7 antibody
Anti-HDAC7B antibody
Anti-HDRP antibody
Anti-KIAA0744 antibody
Anti-MITR antibody

Information sourced from Uniprot.org

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