| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: | HCST |
| Gene ID: | 10870 |
| Uniprot ID: | HCST_HUMAN |
| Immunogen Region: | 1-93 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human HCST (NP_055081.1). |
| Immunogen Sequence: | MIHLGHILFLLLLPVAAAQT TPGERSSLPAFYPGTSGSCS GCGSLSLPLLAGLVAADAVA SLLIVGAVFLCARPRRSPAQ EDGKVYINMPGRG |
| Tissue Specificity | Predominantly expressed in hemopoietic cells such as NK cells, subset of T-cells and monocytes. Detected in leukocytes, spleen, and thymus. |
| Post Translational Modifications | Phosphorylated.PIK3R1 and GRB2 associate specifically with tyrosine-phosphorylated HCST. O-glycosylated. |
| Function | Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells.this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs).these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as a docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells.indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells. |
| Protein Name | Hematopoietic Cell Signal Transducer Dnax-Activation Protein 10 Membrane Protein Dap10 Transmembrane Adapter Protein Kap10 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Hematopoietic Cell Signal Transducer antibody Anti-Dnax-Activation Protein 10 antibody Anti-Membrane Protein Dap10 antibody Anti-Transmembrane Adapter Protein Kap10 antibody Anti-HCST antibody Anti-DAP10 antibody Anti-KAP10 antibody Anti-PIK3AP antibody Anti-UNQ587 antibody Anti-PRO1157 antibody |
Information sourced from Uniprot.org