• Immunofluorescence analysis of A549 cells using HAX1 antibody (STJ27497). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using HAX1 Rabbit polyclonal antibody (STJ27497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HAX1 antibody (1-279) (STJ27497)

SKU:
STJ27497

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAX1 (1-279) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAX1
Gene ID: 10456
Uniprot ID: HAX1_HUMAN
Immunogen Region: 1-279
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human HAX1 (NP_006109.2).
Immunogen Sequence: MSLFDLFRGFFGFPGPRSHR DPFFGGMTRDEDDDEEEEEE GGSWGRGNPRFHSPQHPPEE FGFGFSFSPGGGIRFHDNFG FDDLVRDFNSIFSDMGAWTL PSHPPELPGPESETPGERLR EGQTLRDSMLKYPDSHQPRI FGGVLESDARSESPQPAPDW GSQRPFHRFDDVWPMDPHPR TREDNDLDSQVSQEGLGPVL QPQPKSYFKSISVTKITKPD GIVEERRTVVDSEGRTETT
Tissue Specificity Ubiquitous. Up-regulated in oral cancers.
Post Translational Modifications Proteolytically cleaved by caspase-3 during apoptosis.
Function Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex. Slows down the rate of inactivation of KCNC3 channels. Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.
Protein Name Hcls1-Associated Protein X-1
Hs1-Associating Protein X-1
Hax-1
Hs1-Binding Protein 1
Hsp1bp-1
Cellular Localisation Mitochondrion Matrix
Endoplasmic Reticulum
Nucleus Membrane
Cytoplasmic Vesicle
Cytoplasm
Cell Cortex
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Sarcoplasmic Reticulum
P-Body
Isoform 1: Cytoplasm
Nucleus
Predominantly Cytoplasmic
Also Detected In The Nucleus When Nuclear Export Is Inhibited
And In Response To Cellular Stress Caused By Arsenite (In Vitro)
Isoform 3: Cytoplasm
Also Detected In The Nucleus When Nuclear Export Is Inhibited (In Vitro)
Isoform 4: Cytoplasm
Shuttles Between Nucleus And Cytoplasm
Isoform 5: Cytoplasm
Alternative Antibody Names Anti-Hcls1-Associated Protein X-1 antibody
Anti-Hs1-Associating Protein X-1 antibody
Anti-Hax-1 antibody
Anti-Hs1-Binding Protein 1 antibody
Anti-Hsp1bp-1 antibody
Anti-HAX1 antibody
Anti-HS1BP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance