• Western blot analysis of extracts of various cell lines, using HARS antibody (STJ110208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using HARS antibody (STJ110208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HARS antibody (1-180) (STJ110208)

SKU:
STJ110208

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HARS (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HARS1
Gene ID: 3035
Uniprot ID: HARS1_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HARS (NP_002100.2).
Immunogen Sequence: MAERAALEELVKLQGERVRG LKQQKASAELIEEEVAKLLK LKAQLGPDESKQKFVLKTPK GTRDYSPRQMAVREKVFDVI IRCFKRHGAEVIDTPVFELK ETLMGKYGEDSKLIYDLKDQ GGELLSLRYDLTVPFARYLA MNKLTNIKRYHIAKVYRRDN PAMTRGRYREFYQCDFDIAG
Tissue Specificity Brain, heart, liver and kidney.
Function Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.
Protein Name Histidine--Trna Ligase - Cytoplasmic
Histidyl-Trna Synthetase
Hisrs
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Histidine--Trna Ligase - Cytoplasmic antibody
Anti-Histidyl-Trna Synthetase antibody
Anti-Hisrs antibody
Anti-HARS1 antibody
Anti-HARS antibody
Anti-HRS antibody

Information sourced from Uniprot.org

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