• Western blot analysis of extracts of various cell lines, using HAND1 antibody (STJ111897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using HAND1 antibody (STJ111897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HAND1 antibody (16-215) (STJ111897)

SKU:
STJ111897

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAND1 (16-215) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAND1
Gene ID: 9421
Uniprot ID: HAND1_HUMAN
Immunogen Region: 16-215
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 16-215 of human HAND1 (NP_004812.1).
Immunogen Sequence: PHPAHPMLHEPFLFGPASRC HQERPYFQSWLLSPADAAPD FPAGGPPPAAAAAATAYGPD ARPGQSPGRLEALGGRLGRR KGSGPKKERRRTESINSAFA ELRECIPNVPADTKLSKIKT LRLATSYIAYLMDVLAKDAQ SGDPEAFKAELKKADGGRES KRKRELQQHEGFPPALGPVE KRIKGRTGWPQQVWALELNQ
Tissue Specificity Heart.
Post Translational Modifications Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to translocation into the nucleoplasm, allowing dimerization and transcription factor activity.
Function Transcription factor that plays an essential role in both trophoblast giant cell differentiation and in cardiac morphogenesis. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box). Acts as a transcriptional repressor of SOX15. In the adult, could be required for ongoing expression of cardiac-specific genes.
Protein Name Heart- And Neural Crest Derivatives-Expressed Protein 1
Class A Basic Helix-Loop-Helix Protein 27
Bhlha27
Extraembryonic Tissues - Heart - Autonomic Nervous System And Neural Crest Derivatives-Expressed Protein 1
Ehand
Database Links Reactome: R-HSA-9733709
Cellular Localisation Nucleus
Nucleoplasm
Nucleolus
Interaction With Mdfic Sequesters It Into The Nucleolus
Preventing The Transcription Factor Activity
Phosphorylation By Plk4 Disrupts The Interaction With Mdfic And Releases It From The Nucleolus
Leading To Transcription Factor Activity
Alternative Antibody Names Anti-Heart- And Neural Crest Derivatives-Expressed Protein 1 antibody
Anti-Class A Basic Helix-Loop-Helix Protein 27 antibody
Anti-Bhlha27 antibody
Anti-Extraembryonic Tissues - Heart - Autonomic Nervous System And Neural Crest Derivatives-Expressed Protein 1 antibody
Anti-Ehand antibody
Anti-HAND1 antibody
Anti-BHLHA27 antibody
Anti-EHAND antibody

Information sourced from Uniprot.org

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