• Western blot analysis of extracts from normal (control) and HAGH knockout (KO) HeLa cells, using HAGH antibody (STJ11100791) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HAGH antibody (1-260) (STJ11100791)

SKU:
STJ11100791

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAGH (1-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAGH
Gene ID: 3029
Uniprot ID: GLO2_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HAGH (NP_001035517.1).
Immunogen Sequence: MKVEVLPALTDNYMYLVIDD ETKEAAIVDPVQPQKVVDAA RKHGVKLTTVLTTHHHWDHA GGNEKLVKLESGLKVYGGDD RIGALTHKITHLSTLQVGSL NVKCLATPCHTSGHICYFVS KPGGSEPPAVFTGDTLFVAG CGKFYEGTADEMCKALLEVL GRLPPDTRVYCGHEYTINNL KFARHVEPGNAAIREKLAWA KEKYSIGEPTVPSTLAEEFT YNPFMRVREKTVQQHAGET
Tissue Specificity Expressed in liver and kidney.
Function Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
Protein Name Hydroxyacylglutathione Hydrolase - Mitochondrial
Glyoxalase Ii
Glx Ii
Database Links Reactome: R-HSA-70268 Q16775-2
Cellular Localisation Isoform 1: Mitochondrion Matrix
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Hydroxyacylglutathione Hydrolase - Mitochondrial antibody
Anti-Glyoxalase Ii antibody
Anti-Glx Ii antibody
Anti-HAGH antibody
Anti-GLO2 antibody
Anti-HAGH1 antibody

Information sourced from Uniprot.org

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