Anti-H1-2 antibody (100-200) (STJ11105680)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Histone H1.2 (100-200) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | H1-2 |
| Gene ID | 3006 |
| Uniprot ID | H12_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | GASGSFKLNKKAASGEAKPK VKKAGGTKPKKPVGAAKKPK KAAGGATPKKSAKKTPKKAK KPAAATVTKKVAKSPKKAKV AKPKKAAKSAAKAVKPKAAK P |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HIST1H1C (NP_005310.1). |
Additional Info
| Post Translational Modifications | H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. ADP-ribosylated on Ser-188 in response to DNA damage. |
| Function | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. |
| Protein Name | Histone H1.2Histone H1cHistone H1dHistone H1s-1 |
| Database Links | Reactome: R-HSA-140342Reactome: R-HSA-2559584 |
| Cellular Localisation | NucleusChromosomeMainly Localizes In EuchromatinDistribution Goes In Parallel With Dna Concentration |
| Alternative Antibody Names | Anti-Histone H1.2 antibodyAnti-Histone H1c antibodyAnti-Histone H1d antibodyAnti-Histone H1s-1 antibodyAnti-H1-2 antibodyAnti-H1F2 antibodyAnti-HIST1H1C antibody |
Information sourced from Uniprot.org