Immunofluorescence analysis of U2OS cells using HIST1H1A Rabbit polyclonal antibody (STJ11103985) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-H1-1 antibody (116-168) (STJ11103985)

SKU:
STJ11103985
£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-1
Gene ID: 3024
Uniprot ID: H11_HUMAN
Immunogen Region: 116-168
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 116-168 of human HIST1H1A (NP_005316.1).
Immunogen Sequence: SVETKPGASKVATKTKATGA SKKLKKATGASKKSVKTPKK AKKPAATRKSSKN
Post Translational Modifications H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
Protein Name Histone H1.1
Histone H1a
Database Links Reactome: R-HSA-140342
Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Chromosome
Mainly Localizes In Euchromatin
Alternative Antibody Names Anti-Histone H1.1 antibody
Anti-Histone H1a antibody
Anti-H1-1 antibody
Anti-H1F1 antibody
Anti-HIST1H1A antibody

Information sourced from Uniprot.org

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