• Western blot analysis of over-expressed GST protein using GST-tag antibody (STJ11103698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of over-expressed GST protein using GST-Tag antibody (STJ11103698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 50 Mu g extracts of GST Protein using 3 Mu g GST-Tag antibody (STJ11103698). Western blot was performed from the immunoprecipitate using GST-Tag antibody (STJ11103698).
  • Immunoprecipitation analysis of 300 Mu g extracts of GST-protein cells using 3 Mu g rabbit anti GST-Tag antibody (STJ11103698). Western blot was performed from the immunoprecipitate using rabbit anti GST-Tag antibody (STJ11103698) at a dilution of 1:10000.

Anti-GST-Tag antibody (1-218) (STJ11103698)

SKU:
STJ11103698

£109.50 - £140.50
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GST-Tag (1-218) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IP 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-218
Immunogen: Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
Immunogen Sequence: MSPILGYWKIKGLVQPTRLL LEYLEEKYEEHLYERDEGDK WRNKKFELGLEFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKV DFLSKLPEMLKMFEDRLCHK TYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFK KRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPK
Background Enables several functions, including ATPase binding activity; angiostatin binding activity; and mitochondrial proton-transporting ATP synthase complex binding activity. Involved in several processes, including mitochondrial depolarization; negative regulation of ATPase activity; and regulation of protein targeting to mitochondrion. Located in cell surface and mitochondrion.

Information sourced from Uniprot.org

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