• Western blot analysis of recombinant GST protein using HRP-conjugated mouse anti GST-Tag antibody (STJ11103703) at dilution of 1:5000. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Western blot analysis of recombinant GST Protein using HRP-conjugated Mouse anti GST-Tag monoclonal antibody (STJ11103703) at 1:5000 dilution. Lysates/proteins: 20 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GST-Tag antibody (1-218) [S3MM] {HRP} (STJ11103703)

SKU:
STJ11103703-100

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Host: Mouse
Applications: WB
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-GST-Tag (1-218) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S3MM
Conjugation: HRP
Isotype: igg2bk
Formulation: PBS with 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-218
Immunogen: Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
Immunogen Sequence: MSPILGYWKIKGLVQPTRLL LEYLEEKYEEHLYERDEGDK WRNKKFELGLEFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKV DFLSKLPEMLKMFEDRLCHK TYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFK KRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPK
Background Predicted to enable DNA binding activity. Predicted to be involved in regulation of gene expression. Predicted to act upstream of or within negative regulation of protein sumoylation; positive regulation of neuron differentiation; and skeletal muscle cell differentiation. Located in nuclear body.

Information sourced from Uniprot.org

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