| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
Gsdmc2 |
| Gene ID: |
331063 |
| Uniprot ID: |
GSDC2_MOUSE |
| Immunogen Region: |
367-467 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 367-467 of mouse Gsdmc2 (NP_001161746.1). |
| Immunogen Sequence: |
DSQLNLLAQSVEMGILPHQV ELVKSILQPNFKYPWNIPFT LQPQLLAPLQGEGLAITYEL LEECGLKMELNNPRSTWDLE AKMPLSALYGSLSFLQQLRK A |
| Post Translational Modifications | Cleavage by CASP8 relieves autoinhibition by releasing the N-terminal moiety (Gasdermin-C2, N-terminal) that initiates pyroptosis. Palmitoylated. |
| Function | Gasdermin-C2: This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-C2, N-terminal) binds to membranes and forms pores, triggering pyroptosis. Gasdermin-C2, N-terminal: Pore-forming protein that causes membrane permeabilization and pyroptosis in response to type-2 immunity. Produced by the cleavage of gasdermin-C2 in response to type-2 immunity following worm infection. After cleavage, moves to the plasma membrane where it strongly binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis and lytic cell death in enterocytes. |
| Protein Name | Gasdermin-C2 Cleaved Into - Gasdermin-C2 - N-TerminalGsdmc2-Nt - Gasdermin-C2 - C-TerminalGsdmc2-Ct |
| Cellular Localisation | Gasdermin-C2: CytoplasmCytosolGasdermin-C2N-Terminal: Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Gasdermin-C2 Cleaved Into - Gasdermin-C2 - N-Terminal antibodyAnti-Gsdmc2-Nt - Gasdermin-C2 - C-Terminal antibodyAnti-Gsdmc2-Ct antibodyAnti-Gsdmc2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SMAD2 knockout (KO) Hela (KO) cells, using [KO Validated] Smad2 Rabbit monoclonal antibody (STJ11104898) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/177542/369359/STJ11104898_1__63390.1713134857.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SMAD2 knockout (KO) Hela (KO) cells, using [KO Validated] Smad2 Rabbit monoclonal antibody (STJ11104898) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
