• Western blot analysis of lysates from RAW 264. 7 cells using GSDMC Rabbit monoclonal antibody (STJ11105574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-GSDMC antibody (1-150) [S5574RM] (STJ11105574)

SKU:
STJ11105574

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Gasdermin-C (1-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5574RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSDMC
Gene ID: 56169
Uniprot ID: GSDMC_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GSDMC (NP_113603.1).
Immunogen Sequence: MPSMLERISKNLVKEIGSKD LTPVKYLLSATKLRQFVILR KKKDSRSSFWEQSDYVPVEF SLNDILEPSSSVLETVVTGP FHFSDIMIQKHKADMGVNVG IEVSVSGEASVDHGCSLEFQ IVTIPSPNLEDFQKRKLLDP EPSFLKECRR
Tissue Specificity Expressed mainly in trachea and spleen. In the esophagus, expressed in differentiating cells and probably in differentiated cells. Also detected in gastric epithelium.
Post Translational Modifications Cleavage by CASP8 relieves autoinhibition by releasing the N-terminal moiety (Gasdermin-C, N-terminal) that initiates pyroptosis. The cleavage site is unclear. According to a publication, it takes place after Asp-240 in response to alpha-ketoglutarate. Another paper reports cleavage by CASP8 after Asp-365.
Function Gasdermin-C: This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-C, N-terminal) binds to membranes and forms pores, triggering pyroptosis. Gasdermin-C, N-terminal: Pore-forming protein that causes membrane permeabilization and pyroptosis. Produced by the cleavage of gasdermin-D by caspase CASP8 in response to death signals. After cleavage, moves to the plasma membrane where it strongly binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis.
Protein Name Gasdermin-C
Melanoma-Derived Leucine Zipper-Containing Extranuclear Factor Cleaved Into - Gasdermin-C - N-Terminal
Gsdmc-Nt - Gasdermin-C - C-Terminal
Gsdmc-Ct
Cellular Localisation Gasdermin-C: Cytoplasm
Cytosol
Gasdermin-C
N-Terminal: Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Gasdermin-C antibody
Anti-Melanoma-Derived Leucine Zipper-Containing Extranuclear Factor Cleaved Into - Gasdermin-C - N-Terminal antibody
Anti-Gsdmc-Nt - Gasdermin-C - C-Terminal antibody
Anti-Gsdmc-Ct antibody
Anti-GSDMC antibody
Anti-MLZE antibody

Information sourced from Uniprot.org

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