• Western blot analysis of extracts of various cell lines, using GRM5 antibody (STJ111861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of mouse brain cells using GRM5 rabbit polyclonal antibody (STJ111861) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using GRM5 rabbit polyclonal antibody (STJ111861) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GRM5 antibody (870-1212) (STJ111861)

SKU:
STJ111861

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRM5
Gene ID: 2915
Uniprot ID: GRM5_HUMAN
Immunogen Region: 870-1212
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 870-1070 of human GRM5 (NP_001137303.1).
Immunogen Sequence: GSSGETLRYKDRRLAQHKSE IECFTPKGSMGNGGRATMSS SNGKSVTWAQNEKSSRGQHL WQRLSIHINKKENPNQTAVI KPFPKSTESRGLGAGAGAGG SAGGVGATGGAGCAGAGPGG PESPDAGPKALYDVAEAEEH FPAPARPRSPSPISTLSHRA GSASRTDDDVPSLHSEPVAR SSSSQGSLMEQISSVVTRFT A
Function G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Protein Name Metabotropic Glutamate Receptor 5
Mglur5
Database Links Reactome: R-HSA-416476
Reactome: R-HSA-420499
Reactome: R-HSA-6794361
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Metabotropic Glutamate Receptor 5 antibody
Anti-Mglur5 antibody
Anti-GRM5 antibody
Anti-GPRC1E antibody
Anti-MGLUR5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance