Anti-GPR183 antibody (Internal) (STJ72436)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-GPR183 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/IF/FC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.IF: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml. FC:Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | GPR183 |
| Gene ID | 1880 |
| Uniprot ID | GP183_HUMAN |
| Accession Number | NP_004942.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | NKIKRIEHAK |
Additional Info
| Function | G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes. Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses. Collaborates with CXCR5 to mediate B-cell migration.probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5. Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand. Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand. Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4(+) dendritic cells in bridging channels. Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages. Promotes osteoclast precursor migration to bone surfaces. Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2). |
| Protein Name | G-Protein Coupled Receptor 183Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2Ebi2Ebv-Induced G-Protein Coupled Receptor 2Hebi2 |
| Database Links | Reactome: R-HSA-373076Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-G-Protein Coupled Receptor 183 antibodyAnti-Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2 antibodyAnti-Ebi2 antibodyAnti-Ebv-Induced G-Protein Coupled Receptor 2 antibodyAnti-Hebi2 antibodyAnti-GPR183 antibodyAnti-EBI2 antibody |
Information sourced from Uniprot.org