• Immunofluorescence analysis of NIH-3T3 cells using glucose-6-phosphate isomerase Rabbit polyclonal antibody (STJ115272) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using glucose-6-phosphate isomerase Rabbit polyclonal antibody (STJ115272) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GPI antibody (1-280) (STJ115272)

SKU:
STJ115272

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPI (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPI
Gene ID: 2821
Uniprot ID: G6PI_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human glucose-6-phosphate isomerase (NP_000166.2).
Immunogen Sequence: MAALTRDPQFQKLQQWYREH RSELNLRRLFDANKDRFNHF SLTLNTNHGHILVDYSKNLV TEDVMRMLVDLAKSRGVEAA RERMFNGEKINYTEGRAVLH VALRNRSNTPILVDGKDVMP EVNKVLDKMKSFCQRVRSGD WKGYTGKTITDVINIGIGGS DLGPLMVTEALKPYSSGGPR VWYVSNIDGTHIAKTLAQLN PESSLFIIASKTFTTQETIT NAETAKEWFLQAAKDPSAV
Post Translational Modifications Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway. ISGylated.
Function In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
Protein Name Glucose-6-Phosphate Isomerase
Gpi
Autocrine Motility Factor
Amf
Neuroleukin
Nlk
Phosphoglucose Isomerase
Pgi
Phosphohexose Isomerase
Phi
Sperm Antigen 36
Sa-36
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Secreted
Alternative Antibody Names Anti-Glucose-6-Phosphate Isomerase antibody
Anti-Gpi antibody
Anti-Autocrine Motility Factor antibody
Anti-Amf antibody
Anti-Neuroleukin antibody
Anti-Nlk antibody
Anti-Phosphoglucose Isomerase antibody
Anti-Pgi antibody
Anti-Phosphohexose Isomerase antibody
Anti-Phi antibody
Anti-Sperm Antigen 36 antibody
Anti-Sa-36 antibody
Anti-GPI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance