• Confocal imaging of Hep G2 cells using Glypican 3 (GPC3) Rabbit monoclonal antibody (STJ11101389, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of lysates from HepG2 cells, using Glypican 3 (GPC3) (GPC3) Rabbit monoclonal antibody (STJ11101389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GPC3 antibody (21-211) [S9MR] (STJ11101389)

SKU:
STJ11101389

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Glypican 3 (21-211) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPC3
Gene ID: 2719
Uniprot ID: GPC3_HUMAN
Immunogen Region: 21-211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-211 of human Glypican 3 (GPC3) (P51654).
Immunogen Sequence: PGQAQPPPPPPDATCHQVRS FFQRLQPGLKWVPETPVPGS DLQVCLPKGPTCCSRKMEEK YQLTARLNMEQLLQSASMEL KFLIIQNAAVFQEAFEIVVR HAKNYTNAMFKNNYPSLTPQ AFEFVGEFFTDVSLYILGSD INVDDMVNELFDSLFPVIYT QLMNPGLPDSALDINECLRG ARRDLKVFGNF
Tissue Specificity Detected in placenta (at protein level). Highly expressed in lung, liver and kidney.
Post Translational Modifications O-glycosylated.contains heparan sulfate and/or chondroitin sulfate. Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor. This processing is essential for its role in inhibition of hedgehog signaling. A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface.
Function Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.
Protein Name Glypican-3
Gtr2-2
Intestinal Protein Oci-5
Mxr7 Cleaved Into - Glypican-3 Alpha Subunit - Glypican-3 Beta Subunit
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-381426
Reactome: R-HSA-4420332
Reactome: R-HSA-8957275
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Alternative Antibody Names Anti-Glypican-3 antibody
Anti-Gtr2-2 antibody
Anti-Intestinal Protein Oci-5 antibody
Anti-Mxr7 Cleaved Into - Glypican-3 Alpha Subunit - Glypican-3 Beta Subunit antibody
Anti-GPC3 antibody
Anti-OCI5 antibody

Information sourced from Uniprot.org

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