• Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L. ) variety Zhonghua 11, using Gn1a Rabbit polyclonal antibody (STJ11104056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Gn1a antibody (400-500 aa) (STJ11104056)

SKU:
STJ11104056

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Host: Rabbit
Applications: WB/ELISA
Reactivity: O.sativa
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 400-500 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of Oryza sativa Gn1a. (Q4ADV8).
Immunogen Sequence: RVHDGELKLRAAGLWDVPHP WLNLFLPRSGVLAFADGVFH GILSRTPAMGPVLIYPMNRN KWDSNMSAVITDDDGDEVFY TVGILRSAAAAGDVGRLEEQ N
Background is a gene for cytokinin oxidase/dehydrogenase (OsCKX2) , an enzyme that degrades the phytohormone cytokinin.Reduced expression of OsCKX2 causes cytokinin accumulation in inflorescence meristems and increases the number of reproductive organs, resulting in enhanced grain yield.

Information sourced from Uniprot.org

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