• Immunohistochemistry analysis of paraffin-embedded mouse testis using Glycerol kinase (GK) Rabbit monoclonal antibody (STJ11102954) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Glycerol kinase (GK) Rabbit monoclonal antibody (STJ11102954) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Glycerol kinase (GK) (GK) antibody (STJ11102954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using Glycerol kinase (GK) (GK) antibody (STJ11102954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Glycerol kinase antibody (450-559) [S4MR] (STJ11102954)

SKU:
STJ11102954

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Glycerol kinase (GK) (450-559) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GK
Gene ID: 2710
Uniprot ID: GLPK_HUMAN
Immunogen Region: 450-559
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 450-559 of human Glycerol kinase (GK) (P32189).
Immunogen Sequence: IPVVKPSMPETTALGAAMAA GAAEGVGVWSLEPEDLSAVT MERFEPQINAEESEIRYSTW KKAVMKSMGWVTTQSPESGD PSIFCSLPLGFFIVSSMVML IGARYISGIP
Tissue Specificity Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
Function Key enzyme in the regulation of glycerol uptake and metabolism.
Protein Name Glycerol Kinase
Gk
Glycerokinase
Atp -Glycerol 3-Phosphotransferase
Database Links Reactome: R-HSA-75109
Cellular Localisation Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
In Sperm And Fetal Tissues
The Majority Of The Enzyme Is Bound To Mitochondria
But In Adult Tissues
Such As Liver Found In The Cytoplasm
Alternative Antibody Names Anti-Glycerol Kinase antibody
Anti-Gk antibody
Anti-Glycerokinase antibody
Anti-Atp -Glycerol 3-Phosphotransferase antibody
Anti-GK antibody

Information sourced from Uniprot.org

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