• Immunofluorescence analysis of A-549 cells using GLO1 antibody (STJ23800). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using GLO1 antibody (STJ23800) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GLO1 antibody (STJ23800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-GLO1 antibody (1-184) (STJ23800)

SKU:
STJ23800

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GLO1 (1-184) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GLO1
Gene ID: 2739
Uniprot ID: LGUL_HUMAN
Immunogen Region: 1-184
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human GLO1 (NP_006699.2).
Immunogen Sequence: MAEPQPPSGGLTDEAALSCC SDADPSTKDFLLQQTMLRVK DPKKSLDFYTRVLGMTLIQK CDFPIMKFSLYFLAYEDKND IPKEKDEKIAWALSRKATLE LTHNWGTEDDETQSYHNGNS DPRGFGHIGIAVPDVYSACK RFEELGVKFVKKPDDGKMKG LAFIQDPDGYWIEILNPNKM ATLM
Post Translational Modifications Glutathionylation at Cys-139 inhibits enzyme activity. Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B. Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.
Function Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.
Protein Name Lactoylglutathione Lyase
Aldoketomutase
Glyoxalase I
Glx I
Ketone-Aldehyde Mutase
Methylglyoxalase
S-D-Lactoylglutathione Methylglyoxal Lyase
Database Links Reactome: R-HSA-70268
Alternative Antibody Names Anti-Lactoylglutathione Lyase antibody
Anti-Aldoketomutase antibody
Anti-Glyoxalase I antibody
Anti-Glx I antibody
Anti-Ketone-Aldehyde Mutase antibody
Anti-Methylglyoxalase antibody
Anti-S-D-Lactoylglutathione Methylglyoxal Lyase antibody
Anti-GLO1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance