• STJ73750 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73750 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-GLI1 antibody (Internal) (STJ73750)

SKU:
STJ73750-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-GLI1 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of U2OS cells. 10µg/ml
FC-Flow cytometric analysis of HepG2 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: GLI1
Gene ID: 2735
Uniprot ID: GLI1_HUMAN
Immunogen Region: Internal
Accession Number: NP_005260.1; NP_001153517.1; NP_001161081.1
Specificity: This antibody is expected to recognize the reported isoforms (NP_005260.1; NP_001153517.1; NP_001161081.1).
Immunogen Sequence: TYSQCPRLEHYGQ
Post Translational Modifications Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1. Ubiquitinated by the CRL2(FEM1B) complex, suppressing GLI1 transcriptional activator activity.
Function Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling. Isoform 2: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
Protein Name Zinc Finger Protein Gli1
Glioma-Associated Oncogene
Oncogene Gli
Database Links Reactome: R-HSA-5610780
Reactome: R-HSA-5610787
Reactome: R-HSA-5632684
Reactome: R-HSA-5635851
Cellular Localisation Cytoplasm
Nucleus
Tethered In The Cytoplasm By Binding To Sufu
Activation And Translocation To The Nucleus Is Promoted By Interaction With Stk36
Phosphorylation By Ulk3 May Promote Nuclear Localization
Translocation To The Nucleus Is Promoted By Interaction With Zic1
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Zinc Finger Protein Gli1 antibody
Anti-Glioma-Associated Oncogene antibody
Anti-Oncogene Gli antibody
Anti-GLI1 antibody
Anti-GLI antibody

Information sourced from Uniprot.org

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