• Western blot analysis of extracts of MCF7 cells, using GATC antibody (STJ116100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-GATC antibody (1-136) (STJ116100)

SKU:
STJ116100

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GATC (1-136) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATC
Gene ID: 283459
Uniprot ID: GATC_HUMAN
Immunogen Region: 1-136
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human GATC (NP_789788.1).
Immunogen Sequence: MWSRLVWLGLRAPLGGRQGF TSKADPQGSGRITAAVIEHL ERLALVDFGSREAVARLEKA IAFADRLRAVDTDGVEPMES VLEDRCLYLRSDNVVEGNCA DELLQNSHRVVEEYFVAPPG NISLPKLDEQEPFPHS
Function Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Protein Name Glutamyl-Trna(Gln Amidotransferase Subunit C - Mitochondrial
Glu-Adt Subunit C
Protein 15e1.2
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Glutamyl-Trna(Gln Amidotransferase Subunit C - Mitochondrial antibody
Anti-Glu-Adt Subunit C antibody
Anti-Protein 15e1.2 antibody
Anti-GATC antibody
Anti-15E1.2 antibody

Information sourced from Uniprot.org

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