• Western blot analysis of extracts from wild type (WT) and G6PD knockdown (KD) HeLa cells, using G6PD antibody (STJ11104516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-G6PD antibody (195-515) (STJ11104516)

SKU:
STJ11104516

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Glucose-6-Phosphate 1-Dehydrogenase (195-515) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: G6PD
Gene ID: 2539
Uniprot ID: G6PD_HUMAN
Immunogen Region: 195-515
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 195-515 of human G6PD (NP_001035810.1).
Immunogen Sequence: QIYRIDHYLGKEMVQNLMVL RFANRIFGPIWNRDNIACVI LTFKEPFGTEGRGGYFDEFG IIRDVMQNHLLQMLCLVAME KPASTNSDDVRDEKVKVLKC ISEVQANNVVLGQYVGNPDG EGEATKGYLDDPTVPRGSTT ATFAAVVLYVENERWDGVPF ILRCGKALNERKAEVRLQFH DVAGDIFHQQCKRNELVIRV QPNEAVYTKMMTKKPGMFFN PEESELDLTYGNRYKNVKL
Tissue Specificity Isoform Long is found in lymphoblasts, granulocytes and sperm.
Post Translational Modifications Acetylated by ELP3 at Lys-403.acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403.deacetylation stimulates its enzyme activity.
Function Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Protein Name Glucose-6-Phosphate 1-Dehydrogenase
G6pd
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-71336
Reactome: R-HSA-9818028
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Glucose-6-Phosphate 1-Dehydrogenase antibody
Anti-G6pd antibody
Anti-G6PD antibody

Information sourced from Uniprot.org

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