• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g FTO antibody (STJ11101960). Western blot was performed from the immunoprecipitate using FTO antibody (STJ11101960) at a dilution of 1:500.
  • Confocal imaging of U-2 OS cells using FTO Rabbit monoclonal antibody (STJ11101960, dilution 1:50) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunohistochemistry analysis of FTO in paraffin-embedded human bladder cancer using FTO Rabbit monoclonal antibody (STJ11101960) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FTO Rabbit monoclonal antibody (STJ11101960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-FTO antibody (1-100) [S0MR] (STJ11101960)

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STJ11101960

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-FTO (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FTO
Gene ID: 79068
Uniprot ID: FTO_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FTO (Q9C0B1).
Immunogen Sequence: MKRTPTAEEREREAKKLRLL EELEDTWLPYLTPKDDEFYQ QWQLKYPKLILREASSVSEE LHKEVQEAFLTLHKHGCLFR DLVRIQGKDLLTPVSRILIG
Tissue Specificity Ubiquitously expressed, with relatively high expression in adrenal glands and brain.especially in hypothalamus and pituitary. Highly expressed in highly expressed in acute myeloid leukemias (AML) with t(11.1)(q23.3) with KMT2A/MLL1 rearrangements, t(15.7)(q21.21)/PML-RARA, FLT3-ITD, and/or NPM1 mutations.
Function RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs. Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression.
Protein Name Alpha-Ketoglutarate-Dependent Dioxygenase Fto
Fat Mass And Obesity-Associated Protein
U6 Small Nuclear Rna
2'-O-Methyladenosine-N(6--Demethylase Fto
U6 Small Nuclear Rna N(6-Methyladenosine-Demethylase Fto
Mrna
2'-O-Methyladenosine-N(6--Demethylase Fto
M6a(M-Demethylase Fto
Mrna N(6-Methyladenosine Demethylase Fto
Trna N1-Methyl Adenine Demethylase Fto
Database Links Reactome: R-HSA-73943
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Localizes Mainly In The Nucleus
Where It Is Able To Demethylate N(6)-Methyladenosine (M6a) And N(6)
2'-O-Dimethyladenosine Cap (M6a(M)) In U6 Small Nuclear Rna (Snrna)
N(1)-Methyladenine From Trnas And Internal M6a In Mrnas
In The Cytoplasm
Mediates Demethylation Of M6a And M6a(M) In Mrnas And N(1)-Methyladenine From Trnas
Alternative Antibody Names Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Fto antibody
Anti-Fat Mass And Obesity-Associated Protein antibody
Anti-U6 Small Nuclear Rna antibody
Anti-2'-O-Methyladenosine-N(6--Demethylase Fto antibody
Anti-U6 Small Nuclear Rna N(6-Methyladenosine-Demethylase Fto antibody
Anti-Mrna antibody
Anti-2'-O-Methyladenosine-N(6--Demethylase Fto antibody
Anti-M6a(M-Demethylase Fto antibody
Anti-Mrna N(6-Methyladenosine Demethylase Fto antibody
Anti-Trna N1-Methyl Adenine Demethylase Fto antibody
Anti-FTO antibody
Anti-KIAA1752 antibody

Information sourced from Uniprot.org

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