• Immunofluorescence analysis of NIH/3T3 cells using FNDC5 Rabbit polyclonal antibody (STJ11100078) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using FNDC5 Rabbit polyclonal antibody (STJ11100078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-FNDC5 antibody (1-150) (STJ11100078)

SKU:
STJ11100078

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FNDC5 (1-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FNDC5
Gene ID: 252995
Uniprot ID: FNDC5_HUMAN
Immunogen Region: 1-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-150 of human FNDC5 (NP_715637.2).
Immunogen Sequence: MHPGSPSAWPPRARAALRLW LGCVCFALVQADSPSAPVNV TVRHLKANSAVVSWDVLEDE VVIGFAISQQKKDVRMLRFI QEVNTTTRSCALWDLEEDTE YIVHVQAISIQGQSPASEPV LFKTPREAEKMASKNKDEVT MKEMGRNQQL
Tissue Specificity Widely expressed, with highest levels in heart. Very low expression, if any, in colon, pancreas and spleen.
Post Translational Modifications The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated.
Function Irisin: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue.
Protein Name Fibronectin Type Iii Domain-Containing Protein 5
Fibronectin Type Iii Repeat-Containing Protein 2 Cleaved Into - Irisin
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Peroxisome Membrane
Imported In Peroxisomes Through The Pex5 Receptor Pathway
Irisin: Secreted
Detected In The Blood Of Individuals Subjected To Endurance Exercise
Alternative Antibody Names Anti-Fibronectin Type Iii Domain-Containing Protein 5 antibody
Anti-Fibronectin Type Iii Repeat-Containing Protein 2 Cleaved Into - Irisin antibody
Anti-FNDC5 antibody
Anti-FRCP2 antibody

Information sourced from Uniprot.org

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