• Western blot analysis of extracts of NIH/3T3 cells, using Fibronectin antibody (STJ29624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-FN1 antibody (1-128) (STJ29624)

SKU:
STJ29624

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Fibronectin (1-128) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FN1
Gene ID: 2335
Uniprot ID: FINC_HUMAN
Immunogen Region: 1-128
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human Fibronectin (NP_002017.1).
Immunogen Sequence: MLRGPGPGLLLLAVQCLGTA VPSTGASKSKRQAQQMVQPQ SPVAVSQSKPGCYDNGKHYQ INQQWERTYLGNALVCTCYG GSRGFNCESKPEAEETCFDK YTGNTYRVGDTYERPKDSMI WDCTCIGA
Tissue Specificity Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
Post Translational Modifications Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia.
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Protein Name Fibronectin
Fn
Cold-Insoluble Globulin
Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1474228
Reactome: R-HSA-1474244
Reactome: R-HSA-1566977
Reactome: R-HSA-202733
Reactome: R-HSA-2129379
Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-354192
Reactome: R-HSA-354194
Reactome: R-HSA-372708
Reactome: R-HSA-381426
Reactome: R-HSA-5674135
Reactome: R-HSA-6785807
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-8874081
Reactome: R-HSA-8957275
Reactome: R-HSA-9634597
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9700645
Reactome: R-HSA-9725370
Reactome: R-HSA-9725371
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Fibronectin antibody
Anti-Fn antibody
Anti-Cold-Insoluble Globulin antibody
Anti-Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 antibody
Anti-FN1 antibody
Anti-FN antibody

Information sourced from Uniprot.org

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