| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FGF2 |
| Gene ID: | 2247 |
| Uniprot ID: | FGF2_HUMAN |
| Immunogen Region: | 143-288 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 143-288 of human FGF2 (NP_001997.5). |
| Immunogen Sequence: | PALPEDGGSGAFPPGHFKDP KRLYCKNGGFFLRIHPDGRV DGVREKSDPHIKLQLQAEER GVVSIKGVCANRYLAMKEDG RLLASKCVTDECFFFERLES NNYNTYRSRKYTSWYVALKR TGQYKLGSKTGPGQKAILFL PMSAKS |
| Tissue Specificity | Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. |
| Post Translational Modifications | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
| Function | Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation. |
| Protein Name | Fibroblast Growth Factor 2 Fgf-2 Basic Fibroblast Growth Factor Bfgf Heparin-Binding Growth Factor 2 Hbgf-2 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1839122 Reactome: R-HSA-1839130 Reactome: R-HSA-190322 Reactome: R-HSA-190370 Reactome: R-HSA-190372 Reactome: R-HSA-190373 Reactome: R-HSA-190375 Reactome: R-HSA-190377 Reactome: R-HSA-2033519 Reactome: R-HSA-2219530 Reactome: R-HSA-2892247 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-5654219 Reactome: R-HSA-5654221 Reactome: R-HSA-5654227 Reactome: R-HSA-5654228 Reactome: R-HSA-5654687 Reactome: R-HSA-5654688 Reactome: R-HSA-5654689 Reactome: R-HSA-5654693 Reactome: R-HSA-5654695 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654710 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5654720 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5655253 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5658623 Reactome: R-HSA-5673001 Reactome: R-HSA-6785807 Reactome: R-HSA-6811558 Reactome: R-HSA-8851708 Reactome: R-HSA-9761174 Reactome: R-HSA-9830364 Reactome: R-HSA-9839397 |
| Cellular Localisation | Secreted Nucleus Exported From Cells By An Endoplasmic Reticulum (Er)/Golgi-Independent Mechanism Unconventional Secretion Of Fgf2 Occurs By Direct Translocation Across The Plasma Membrane Binding Of Exogenous Fgf2 To Fgfr Facilitates Endocytosis Followed By Translocation Of Fgf2 Across Endosomal Membrane Into The Cytosol Nuclear Import From The Cytosol Requires The Classical Nuclear Import Machinery Involving Proteins Kpna1 And Kpnb1 As Well As Cep57 |
| Alternative Antibody Names | Anti-Fibroblast Growth Factor 2 antibody Anti-Fgf-2 antibody Anti-Basic Fibroblast Growth Factor antibody Anti-Bfgf antibody Anti-Heparin-Binding Growth Factor 2 antibody Anti-Hbgf-2 antibody Anti-FGF2 antibody Anti-FGFB antibody |
Information sourced from Uniprot.org