• Immunohistochemistry analysis of paraffin-embedded mouse testis using FGF12 antibody (STJ23653) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using FGF12 antibody (STJ23653) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using FGF12 antibody (STJ23653) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using FGF12 antibody (STJ23653) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-FGF12 antibody (1-181) (STJ23653)

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STJ23653

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FGF12 (1-181) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGF12
Gene ID: 2257
Uniprot ID: FGF12_HUMAN
Immunogen Region: 1-181
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human FGF12 (NP_004104.3).
Immunogen Sequence: MESKEPQLKGIVTRLFSQQG YFLQMHPDGTIDGTKDENSD YTLFNLIPVGLRVVAIQGVK ASLYVAMNGEGYLYSSDVFT PECKFKESVFENYYVIYSST LYRQQESGRAWFLGLNKEGQ IMKGNRVKKTKPSSHFVPKP IEVCMYREPSLHEIGEKQGR SRKSSGTPTMNGGKVVNQDS T
Tissue Specificity Brain, eye and testis.highly expressed in embryonic retina, olfactory epithelium, olfactory bulb, and in a segmental pattern of the body wall.in adult olfactory bulb, less in cerebellum, deep cerebellar nuclei, cortex and multiple midbrain structures.
Function Involved in nervous system development and function. Involved in the positive regulation of voltage-gated sodium channel activity. Promotes neuronal excitability by elevating the voltage dependence of neuronal sodium channel SCN8A fast inactivation.
Protein Name Fibroblast Growth Factor 12
Fgf-12
Fibroblast Growth Factor Homologous Factor 1
Fhf-1
Myocyte-Activating Factor
Database Links Reactome: R-HSA-5576892
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Fibroblast Growth Factor 12 antibody
Anti-Fgf-12 antibody
Anti-Fibroblast Growth Factor Homologous Factor 1 antibody
Anti-Fhf-1 antibody
Anti-Myocyte-Activating Factor antibody
Anti-FGF12 antibody
Anti-FGF12B antibody
Anti-FHF1 antibody

Information sourced from Uniprot.org

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