• Western blot analysis of extracts of U-87MG cells, using FAT4 rabbit polyclonal antibody (STJ11102882) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of C6 cells using FAT4 antibody (STJ11102882) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using FAT4 antibody (STJ11102882) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-FAT4 antibody (4900-4981) (STJ11102882)

SKU:
STJ11102882

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAT4 (4900-4981) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAT4
Gene ID: 79633
Uniprot ID: FAT4_HUMAN
Immunogen Region: 4900-4981
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 4900-4981 of human FAT4 (NP_078858.4).
Immunogen Sequence: GGPVGTQAAAPGTADNTLPM KLGQQAGTFNWDNLLNWGPG FGHYVDVFKDLASLPEKAAA NEEGKAGTTKPVPKDGEAEQ YV
Tissue Specificity Widely expressed. Expressed in fetal brain, infant brain, brain tumor and colorectal cancer.
Function Cadherins are calcium-dependent cell adhesion proteins. FAT4 plays a role in the maintenance of planar cell polarity as well as in inhibition of YAP1-mediated neuroprogenitor cell proliferation and differentiation.
Protein Name Protocadherin Fat 4
Hfat4
Cadherin Family Member 14
Fat Tumor Suppressor Homolog 4
Fat-Like Cadherin Protein Fat-J
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
In The Kidney
Localizes To Primary Cilia
Alternative Antibody Names Anti-Protocadherin Fat 4 antibody
Anti-Hfat4 antibody
Anti-Cadherin Family Member 14 antibody
Anti-Fat Tumor Suppressor Homolog 4 antibody
Anti-Fat-Like Cadherin Protein Fat-J antibody
Anti-FAT4 antibody
Anti-CDHF14 antibody
Anti-FATJ antibody
Anti-Nbla00548 antibody

Information sourced from Uniprot.org

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