| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-FAM21C (991-1320) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WASHC2C |
| Gene ID: |
253725 |
| Uniprot ID: |
WAC2C_HUMAN |
| Immunogen Region: |
991-1320 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 991-1320 of human FAM21C (NP_056077.2). |
| Immunogen Sequence: |
GVSFDLPAQADTLHSANKSR VKMRGKRRPQTRAARRLAAQ ESSEAEDMSVPRGPIAQWAD GAISPNGHRPQLRAASGEDS TEEALAAAAAPWEGGPVPGV DTSPFAKSLGHSRGEADLFD SGDIFSTGTGSQSVERTKPK AKIAENPANPPVGGKAKSPM FPALGEASSDDDLFQSAKPK PAKKTNPFPLLEDEDDLFTD QKVKKNETKSSSQQDVILTT QDIFEDDIFATEAIKPSQK |
| Function | Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission. Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35. Required for the endosomal recruitment of CCC and retriever complexes subunits COMMD1 and CCDC93 as well as the retrievere complex subunit VPS35L. (Microbial infection) Plays a role in fluid-phase endocytosis, a process exploited by vaccinia intracellular mature virus (IMV) to enter cells. As a result, may facilitate the penetration of IMV into cells. |
| Protein Name | Wash Complex Subunit 2cVaccinia Virus Penetration FactorVpef |
| Cellular Localisation | Early Endosome MembraneCell MembranePartially Colocalizes With Rab11aA Recycling Endosome MarkerAssociates With Lipid Raft Microdomains On The Plasma Membrane |
| Alternative Antibody Names | Anti-Wash Complex Subunit 2c antibodyAnti-Vaccinia Virus Penetration Factor antibodyAnti-Vpef antibodyAnti-WASHC2C antibodyAnti-FAM21C antibodyAnti-KIAA0592 antibodyAnti-VPEF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
