| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IHC |
| Reactivity: |
Human/Mouse/Rat/Pig/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-EWS/EWSR1 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-0.3-1µg/mlELISA-antibody detection limit dilution 1:64000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
EWSR1 |
| Gene ID: |
2130 |
| Uniprot ID: |
EWS_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1 |
| Specificity: |
This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1). |
| Immunogen Sequence: |
TSYDQSSYSQQNTYG |
| Post Translational Modifications | Phosphorylated.calmodulin-binding inhibits phosphorylation of Ser-266. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8. |
| Function | Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Protein Name | Rna-Binding Protein EwsEws OncogeneEwing Sarcoma Breakpoint Region 1 Protein |
| Cellular Localisation | NucleusCytoplasmCell MembraneRelocates From Cytoplasm To Ribosomes Upon Ptk2b/Fak2 Activation |
| Alternative Antibody Names | Anti-Rna-Binding Protein Ews antibodyAnti-Ews Oncogene antibodyAnti-Ewing Sarcoma Breakpoint Region 1 Protein antibodyAnti-EWSR1 antibodyAnti-EWS antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
