| Host: | Mouse |
| Applications: | IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Estrogen Receptor is suitable for use in Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | ER-71 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Immunohistology (formalin-fixed: 2-4 µg/ml for 30 min at RT; staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20-30 min followed by cooling at RT for 20 minutes). |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: | GPER1 |
| Gene ID: | 2852 |
| Uniprot ID: | GPER1_HUMAN |
| Immunogen: | A BALB/c mouse was immunized with recombinant human estrogen receptor alpha protein (aa2-185).Fusion partner: SP2/0. |
| Post Translational Modifications | Ubiquitinated.ubiquitination occurs at the plasma membrane and leads to proteasome-mediated degradation. Glycosylated. |
| Function | G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Functions also as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells. |
| Protein Name | G-Protein Coupled Estrogen Receptor 1 Chemoattractant Receptor-Like 2 Flow-Induced Endothelial G-Protein Coupled Receptor 1 Feg-1 G Protein-Coupled Estrogen Receptor 1 G-Protein Coupled Receptor 30 Gpcr-Br Il8-Related Receptor Dry12 Lymphocyte-Derived G-Protein Coupled Receptor Lygpr Membrane Estrogen Receptor Mer |
| Database Links | Reactome: R-HSA-375276 Reactome: R-HSA-418594 Reactome: R-HSA-9634597 |
| Cellular Localisation | Nucleus Cytoplasm Perinuclear Region Cytoskeleton Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Cytoplasmic Vesicle Membrane Early Endosome Recycling Endosome Golgi Apparatus Membrane Golgi Apparatus Trans-Golgi Network Endoplasmic Reticulum Membrane Cell Projection Dendrite Dendritic Spine Membrane Axon Postsynaptic Density Mitochondrion Membrane Colocalized With Bsn To The Active Zone Of Presynaptic Density Colocalized With Dlg4/Psd95 And Neurabin-2 Ppp1r9b In Neuronal Synaptosomes Endocytosed In A Agonist- And Arrestin-Independent Manner Colocalized With Ramp3 And Clathrin-Coated Pits At The Plasma Membrane Colocalized With Transferrin Receptor At The Plasma Membrane And Perinuclear Region Accumulated And Colocalized With Rab11 Proteins In Recycling Endosomes And Trans-Golgi Network (Tgn) But Does Neither Recycle Back To The Cell Surface Nor Traffics To Late Endosome Or Lysosome Colocalized With Calnexin In The Endoplasmic Reticulum Traffics To Intracellular Sites Via Cytokeratin Intermediate Filaments Like Krt7 And Krt8 After Constitutive Endocytosis In Epithelial Cells Colocalized With Egfr In The Nucleus Of Agonist-Induced Cancer-Associated Fibroblasts (Caf) |
| Alternative Antibody Names | Anti-G-Protein Coupled Estrogen Receptor 1 antibody Anti-Chemoattractant Receptor-Like 2 antibody Anti-Flow-Induced Endothelial G-Protein Coupled Receptor 1 antibody Anti-Feg-1 antibody Anti-G Protein-Coupled Estrogen Receptor 1 antibody Anti-G-Protein Coupled Receptor 30 antibody Anti-Gpcr-Br antibody Anti-Il8-Related Receptor Dry12 antibody Anti-Lymphocyte-Derived G-Protein Coupled Receptor antibody Anti-Lygpr antibody Anti-Membrane Estrogen Receptor antibody Anti-Mer antibody Anti-GPER1 antibody Anti-CEPR antibody Anti-CMKRL2 antibody Anti-DRY12 antibody Anti-GPER antibody Anti-GPR30 antibody |
Information sourced from Uniprot.org