• Western blot analysis of extracts of various cell lines, using EPHX2 antibody (STJ116135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-EPHX2 antibody (286-555) (STJ116135)

SKU:
STJ116135

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPHX2 (286-555) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHX2
Gene ID: 2053
Uniprot ID: HYES_HUMAN
Immunogen Region: 286-555
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 286-555 of human EPHX2 (NP_001970.2).
Immunogen Sequence: YRVLAMDMKGYGESSAPPEI EEYCMEVLCKEMVTFLDKLG LSQAVFIGHDWGGMLVWYMA LFYPERVRAVASLNTPFIPA NPNMSPLESIKANPVFDYQL YFQEPGVAEAELEQNLSRTF KSLFRASDESVLSMHKVCEA GGLFVNSPEEPSLSRMVTEE EIQFYVQQFKKSGFRGPLNW YRNMERNWKWACKSLGRKIL IPALMVTAEKDFVLVPQMSQ HMEDWIPHLKRGHIEDCGH
Post Translational Modifications The N-terminus is blocked. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).
Function Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. Bifunctional enzyme. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid. Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates.
Protein Name Bifunctional Epoxide Hydrolase 2 Includes - Cytosolic Epoxide Hydrolase 2
Ceh
Epoxide Hydratase
Soluble Epoxide Hydrolase
Seh - Lipid-Phosphate Phosphatase
Database Links Reactome: R-HSA-2142670
Reactome: R-HSA-9018682
Reactome: R-HSA-9033241
Cellular Localisation Cytoplasm
Peroxisome
Alternative Antibody Names Anti-Bifunctional Epoxide Hydrolase 2 Includes - Cytosolic Epoxide Hydrolase 2 antibody
Anti-Ceh antibody
Anti-Epoxide Hydratase antibody
Anti-Soluble Epoxide Hydrolase antibody
Anti-Seh - Lipid-Phosphate Phosphatase antibody
Anti-EPHX2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance