• Western blot analysis of extracts of HeLa cells, using Eph receptor B3 (Eph receptor B3 (EPHB3) ) antibody (STJ112252) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EPHB3 antibody (779-998) (STJ112252)

SKU:
STJ112252

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPHB3 (779-998) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHB3
Gene ID: 2049
Uniprot ID: EPHB3_HUMAN
Immunogen Region: 779-998
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 779-998 of human Eph receptor B3 (EPHB3) (NP_004434.2).
Immunogen Sequence: LSRFLEDDPSDPTYTSSLGG KIPIRWTAPEAIAYRKFTSA SDVWSYGIVMWEVMSYGERP YWDMSNQDVINAVEQDYRLP PPMDCPTALHQLMLDCWVRD RNLRPKFSQIVNTLDKLIRN AASLKVIASAQSGMSQPLLD RTVPDYTTFTTVGDWLDAIK MGRYKESFVSAGFASFDLVA QMTAEDLLRIGVTLAGHQKK ILSSIQDMRLQMNQTLPVQV
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-614 is required for interaction with SH2 domain-containing proteins. Ubiquitinated by RNF186, mainly through 'Lys-48' and 'Lys-63'-linked polyubiquitin chains.
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Protein Name Ephrin Type-B Receptor 3
Eph-Like Tyrosine Kinase 2
Eph-Like Kinase 2
Embryonic Kinase 2
Ek2
Hek2
Tyrosine-Protein Kinase Tyro6
Database Links Reactome: R-HSA-2682334
Reactome: R-HSA-3928662
Reactome: R-HSA-3928664
Reactome: R-HSA-3928665
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Dendrite
Alternative Antibody Names Anti-Ephrin Type-B Receptor 3 antibody
Anti-Eph-Like Tyrosine Kinase 2 antibody
Anti-Eph-Like Kinase 2 antibody
Anti-Embryonic Kinase 2 antibody
Anti-Ek2 antibody
Anti-Hek2 antibody
Anti-Tyrosine-Protein Kinase Tyro6 antibody
Anti-EPHB3 antibody
Anti-ETK2 antibody
Anti-HEK2 antibody
Anti-TYRO6 antibody

Information sourced from Uniprot.org

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